Analysis of spinacia oleracea chlorophyll and quantification of albumin using spectrophotometry

Abstract

Based on pigmentation analysis perceived in objects, which may be explained through physic-chemical analysis, the ultraviolet-visible (UV-VIS) spectrophotometry is realized in this research, in order to analyze the chlorophyll extracted from Spinacia oleracea, leading to interpret and relate the concepts of light spectrum, absorbance and pigments, through preparation and analysis of absorbance curves. In addition, proteins (albumin) extracted from different cost pasta (spaghetti), by using the Lowry method which uses Folin reagent, obtaining experimental evidence, in these cases the relationship of absorbance terms and concentrations of various substances, through Lambert-Beer’s, which is mathematically deduced.