Evaluation of molasses as a culture medium for the production of lactic acid bacteria

  • Laura Marcela Cárdenas C
  • Javier Andrés Gómez S
  • Mateo Arenas A,
  • Johanna Serna-Jiménez
Keywords: Bioreactor, pH, acidification, carbon source, biomass.

Abstract

Bioprocesses are those processes that involve the manipulation of living organisms or their cellular components as substrates in order to obtain a biomass or product to provide goods or services as an example, the use of bacteria acid lactic acid in biopreservacion, this was proposed as target bacteria produce acid lactic acid in an aerobic bioreactor through a conventional molasses as carbon source and check the development of the micro-organism as biomass, to accomplish this was carried out in two different twinjet with volumes of 7, 5L, 0, 5 L using the same substrate and inoculum I, evaluating chemical variables such as pH, acidity, ºbrix, reducing sugars and absorbance . The results show a correct development of the bacteria metabolism lactic acid observing one acidification of the environment demonstrating in the pH decrease and increase of titrable acidity, also seeing a decrease in sugars reducers interpreting this as the consumption of the carbon of the micro-organism and the realization of a process source fermentation and verifying the biomass growth through units of absorbance and the weight of the produced biomass, and may conclude that there was a proper acid development of bacteria lactic demonstrating that molasses is a source of carbon and a half of crop suitable for the production of this bacterium.

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Published
2019-07-02
How to Cite
Cárdenas C, L. M., Gómez S, J. A., Arenas A, M., & Serna-Jiménez, J. (2019). Evaluation of molasses as a culture medium for the production of lactic acid bacteria. UGCiencia, 24(1), 17-22. https://doi.org/10.18634/ugcj.24v.1i.919
Section
Artículos Resultado de Investigación